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苏州工业园区生物纳米园A4#216
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商品描述
| Description | TheLaminproteinsaremembersoftheintermediatefilamentproteinfamilybutarelocatedinsidethenucleusratherthaninthecytoplasm(1).Thelaminsfunctionasskeletalcomponentstightlyassociatedwiththeinnernuclearmembrane.OriginallytheproteinsofthenuclearCytoskeletonwerenamedLaminA,BandC,fromtoptobottomasvisualizedonSDS-PAGEgels.SubsequentlyitwasfoundthatLaminsAandCwerecodedforbyasinglegene(2),whiletheLaminBbandmaycontaintwoproteinsencodedbytwogenesnowcalledLaminB1andLaminB2.LaminAhasamassofabout74kDawhileLaminCis65kDa.TheLaminAproteinincludes98aminoacidsmissingfromLaminC,whileLaminChasaC-terminal6aminoacidpeptidenotpresentinLaminA.ApartfromtheseregionsLaminAandCareidenticalsothatantibodiesraisedagainsteitherproteinarelikelytocrossreactwiththeother,asisthecasewiththismonoclonal.Laminpolymerizationanddepolymerizationisregulatedbyphosphorylationbycyclindependentproteinkinase1(CDK1),thekeycomponentof"maturationpromotingfactor",thecentralregulatorofcelldivision.ActivityofthiskinaseincreasesduringcelldivisionandisresponsIBLeforthebreakdownofthenuclearlamina.MutationsintheLMNAgeneareassociatedwithseveralserioushumandiseases,includingEmery-Dreifussmusculardystrophy,familialpartiallipodystrophy,limbgirdlemusculardystrophy,dilatedcardiomyopathy,Charcot-Marie-Toothdiseasetype2B1,andHutchinson-Gilfordprogeriasyndrome.ThisfamilyofdiseasesbelongtoalargergroupwhichareoftenreferredtoasLaminopathies,thoughsomelaminopathiesareassociatedindefectsinLaminB1,B2oroneorotherofthenumerousnuclearlaminabindingproteins.AtruncatedversionoflaminA,commonlyknownasprogerin,causesHutchinson-Gilfordprogeriasyndrome,aformofprematureaging(3). |
| BatchNo. | Seeviallabel |
| Unitsize | 100uL |
| Antigen | FulllengthrecombinanthumanLaminC |
| AntibodyType | Polyclonal |
| Producedin | Rabbit |
| Applications | Immunocytochemistry(ICC)andWesternBlotting(WB).Adilutionof1:1,000-1:5,000isrecommendedforWB.Adilutionof1:1,000-1:5,000isrecommendedforICC.Theoptimaldilutionshouldbedeterminedbytheenduser. |
| Specificity | LaminAandLaminC.Theantibodyreactswitha74kDaand65kDabandbyWesternblotonHeLacellextract.IthasalsobeenusedsuccessfullyforimmunocytochemistryonHeLacellcultures. |
| SpeciesAgainst | Human,bovine,porcine,mouseandrat.Itisexpectedthatitwillworkonothermammaltissues. |
| Form | LyophilizedfromPBS.Contains0.5%sodiumazide. |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Fisher,D.Z.,Chaudhary,N.,Blobel,G.CDNAsequencingofnuclearlaminsAandCrevealsprimaryandsecondarystructuralhomologytointermediatefilamentproteins.Proc.Nat.Acad.Sci.83:6450-6454(1986).2.McKeon,F.D.,Kirschner,M.W.,Caput,D.Homologiesinbothprimaryandsecondarystructurebetweennuclearenvelopeandintermediatefilamentproteins.Nature319:463-468(1986).3.Liu,B.andZhou,Z.LaminA/C,laminopathiesandprematureageing.Histol.Histopathol.23:747-763(2006). |
