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商品描述
| Description | MouseNGF(2.5S)wasisolatedfrommousesuBMAxillaryglandsbymethodofMobleyetal(1976)andisaformofbeta-NGFthathasidenticalBIOLOGicalproperties.NGFisknowntoregulatethesurvivalanddevelopmentofcertainsympatheticandsensoryneurons.Itisadimerwith2identicalpolypeptidechainsanddimericmolecularweightofapproximately26,500Da.IsolationandpurificationofNGFfrommousesubmaxillaryglandsyieldspreparationsofNGF(2.5S)withidenticalbiologicalactivitybutwithcleavagesattheaminoterminus(withthelossof8aminoacids)and/oratthecarboxy-terminus(withthelossofarginine).Thesepreparationsarenamed2.5NGF(seereferencebelow). |
| BatchNo. | Seeproductlabel |
| Unitsize | 25µg |
| OtherNames | mouseNGF;betaNGF |
| Accession | NGF_MOUSE |
| Producedin | Mouse |
| MolecularWeight | Thehomodimerhasamolecularweightof~26KDaandthemonomer~13KDaasconfirmedbySDSPAGE. |
| Purity | Greaterthan90%(asdeterminedbySDSelectrophoresis) |
| Biol.activity | Testedbystimulationof8dayoldchickembryodorsalrootganglia. |
| Form | Lyophilisedfrom0.1MaceticacidunderdryN2withvacuum.Appearsasnear-whitepowder.Solutionsterilefilteredpriortodrying. |
| Reconstitution | Reconstitutewiththeadditionof25µlsterilewaterorphosphatebufferedsalineanddivideintosingleusealiquots.Flashfreezealiquotsinadryiceethanolbathandstoreat-20Cto-70Cforbestresults. |
| Storage | Shortterm:-20°C.Avoidrepeatedfreezingandthawing.Longerterm-70C.Seeexpirationdateforshelf-lifeestimates,actualtimesmayvarydependinguponexperimentalconditionsandlaboratoryhandling. |
| ExpiryDate | 12monthsuponpurchasefordrymaterial.Hydratedmaterial,5daysat4C,30days-20C,60days-70CforhighestactivityAvoidrepeatedfreezingandthawing.Useinsulatedstoragecontainersforbestresults. |
| SpecificReferences | CarterDRetal(2016)GlutathionebiosynthesisisupregulatedattheinitiationofMYCN-drivenneuroblastomatumOrigenesis.MolOncol.2016Jun;10(6):866-78. GoodhillGJetal(2015)Thedynamicsofgrowthconemorphology.BMCBiol.2015Feb;13(10). CoulsonEJetal(2015)Neurotrophin-tyrosinekinasereceptorsignaling.UnitedStatesPatentApplication20150344535. Gearing,DPetal(2013)Afullycaninisedanti-NGFmonoclonalantibodyforpainreliefindogs.BMCVetRes.9:226. MatusicaDetal(2013)Anintracellulardomainfragmentofthep75neurotrophinreceptor(p75(NTR))enhancestropomyosinreceptorkinaseA(TrkA)receptorfunction.JBiolChem.2013Apr19;288(16):11144-54. YuanJetal(2013)Optimalityandsaturationinaxonalchemotaxis.NeuralComput.2013Apr;25(4):833-53. SykesAMetal(2012)Theeffectsoftransmembranesequenceanddimerizationoncleavageofthep75neurotrophinreceptorbyγ-secretase.JBiolChem.2012Dec21;287(52):43810-24. E.M.Forbesetal(2012)CalciumandcAMPLevelsInteracttoDetermineAttractionversusRepulsioninAxonGuidance.Neuron.2012May10;74(3):490-503. |
| GeneralReferences | MobleyWC,SchenkerA,ShooterEM(1976)Biochemistry.15(25)pp.5543-52. |
