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Biosensis/Human Stem Cell Factor protein expressed in mammalian cells/PE-1242-2/2 µg

价格
¥2540.00
货号:PE-1242-2
浏览量:127
品牌:Biosensis
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商品描述
DescriptionStemCellFactor(SCF)isahematopoieticgrowthfactorthatstimulatestheproliferationofmastcells. SCFalsomediatescell-celladhesionandactssynergisticallywithothercytokines.TheactionsofSCFareexertedbybindingtotheSCFReceptor(PE-1243-50)whichresultsinreceptordimerization.SCFisatypeImembraneglycoproteinwith5potentialN-linkedglycosylationsites.SolubleSCFisproducedbyproteolyticcleavageoftheextracellulardomain.
BatchNo.Seeproductlabel
Unitsize2µg
OtherNamesKitligand;C-kitligand;Stemcellfactor;SCF;Mastcellgrowthfactor;MGF;KITLG;MGF;SCF;
AccessionP21583SCF_HUMAN;
ProducedinHuman-ADNAsequenceencodingthesignalpeptideandthematureStemCellFactor(SCF)proteinofhumanSCFwasexpressedinmodifiedhuman293cells.
MolecularWeightSCFmigratesasabroadbandbetween20and42kDainSDS-PAGEduetopost-translationalmodifications.UnmodifiedSCFhasapredictedmolecularmassof21.0kDa.SCFseparatesintoanumberofisoformswithapIbetween3.0and7.0in2DPAGEduetopost-translationalmodifications.ThiscompareswiththeunmodifiedSCFwithapredictedpIof5.76.
Purity>95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain
Biol.activityTheED50ofSCFistypically10-20ng/mlasmeasuredinacellproliferationassayusingthehumangrowthfactor-dependentM-07ecellline.
FormPurifiedSCFconsistsof0-50%carbohydratebyweight.
ReconstitutionItisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial.
StorageLyophilizedproductsshouldbestoredat2-8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended.