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Biosensis/Human NT3 protein expressed in mammalian cells/PE-1246-5/5 µg

价格
¥5340.00
货号:PE-1246-5
浏览量:127
品牌:Biosensis
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Description
Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
BatchNo.Seeproductlabel
Unitsize5µg
OtherNamesNeurotrophin3;Neurotrophicfactor;HDNF;Nervegrowthfactor2;NGF-2;NTF2;NGF2;NT-3;
AccessionP20783NTF3_HUMAN;
ProducedinHuman-ADNAsequenceencodingthehumanNT3protein(containingthesignalpeptideandthematureNT3sequence)wasexpressedinmodifiedhuman293cells.
MolecularWeightTheNT3proteinmigratesatapproximately14-15kDainSDS-PAGEandhasapredictedmolecularmassof13.6kDa.NT3separatesintoanumberofisoformswithapIfrom6.6toatleast10in2DPAGEduetopost-translationalmodifications.TheunmodifiedNT3hasapredictedpIof9.2.
Purity>95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain.
Biol.activityTheED50ofNT3istypically0.1-0.2ng/mlasmeasuredinacellproliferationassayusingaTrkBtransfectedPC12cellline.
FormWhenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose.
ReconstitutionItisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial.
StorageLyophilizedproductsshouldbestoredat2to8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended.