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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Human Hepatocyte Growth Factor (HGF) protein expressed in mammalian cells/PE-1241-5/5 µg
商品描述
| Description | Hepatocytegrowthfactor(HGF)isamemberoftheplasminogenrelatedgrowthfactorfamily.TheHGFproteinisadimerformedfromanalphaandbetasubunitlinkedbyadisulfidebond.BothsubunitsoriginatefromtheendoproteolyticcleavageofacommonprecursorwhichisBIOLOGicallyinactive.HGFisexpressedinKupffercellaandsinusoidalendothelialcellsintheliverandpancreas. |
| BatchNo. | Seeproductlabel |
| Unitsize | 5µg |
| OtherNames | HGF;HPTA;Scatterfactor;SF;Hepatocytegrowthfactoralphachain;Hepatocytegrowthfactorbetachain;Hepatopoeitin-A; |
| Accession | P14210HGF_HUMAN; |
| Producedin | Human-ADNAsequenceencodingthecompletehumanHepatocyteGrowthFactorproteinisoform1(comprisingthesignalpeptide,alphaandbetachains)wasexpressedinmodifiedhuman293cells. |
| MolecularWeight | Underreducingconditions,theHGFproteinmigratesasabroadbandbetween80and110kDainSDS-PAGEduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedHGFhasapredictedmonomericmolecularmassof79.7kDa.TheproteinseparatesintoanumberofisoformswithapIbetween6and9in2DPAGE.TheunmodifiedHGFproteinhasapredictedpIof8.2 |
| Purity | >95%,asdeterminedbySDS-PAGEandvisualisedbyCoomassieBrilliantBlue |
| Biol.activity | TheED50ofHGFistypically20-40ng/mlasmeasuredinacellproliferationassayusingthehumanHepG2cellline. |
| Form | TheHGFformulationconsistsof0-30%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
| Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
| Storage | Lyophilisedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
