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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Path
商品描述
| Description | Black-Gold®IIisanovelhaloaurophosphatecomplexwhichlocalisesmyelinwithinthecentralnervoussystem.TheBlackGoldIIReady-to-Dilute(RTD)StainingKitallowsyoutolocalisemyelin,bothindividualfibresandtracts,alongwiththeoptionofco-localisingcellbodiesviatheToluidineBluecounterstain.BlackGoldIIlabelledmyelinatedfibresappearnearlyblackwhiletheToluidineBlueOlabelledcellularNisslbodiesareblueunderbrightfieldIllumination. BlackGoldIIcandemonstrateandcharacterisespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid.BlackGoldIIcanalsobecombinedwithotherhistochemicalMarkersincludingNisslstains,retrogradelytransportedfluorescenttracersandfluorescentmarkersofneuronaldegeneration.TheadvantagesassociatedwiththeBlack-GoldIIincludehighresolution,highcontrast,shorthistochemicalprocessingtime,versatilityandconsistentreproducibility. |
| OtherNames | blackandgold |
| Applications | BlackGoldIIisahighresolutionmyelinstainwithamyloidplaquecounterstain.Itsuseistailoredtostudiesusingformalinorparaformaldehydefixed,non-paraffinembedded,non-solventprocessedbraintissue.Itcanbeusedwithboththickandthinsections.Forthicksections,gelatincoatedslidesorslidesspeciallydesignedtobindtissuessectionsshouldbeusetoavoidsectionloss.Free-floatingsectionscanbeusedaswellbutsectionsareeasiertohandleandtransferwhenmountedonslides.Asuggestedmethodforthicksectionsisprovidedasaguide:Eitherfrozenorvibratomesectionsarecutatathicknessof20-50umandcollectedin0.1Mneutralphosphatebuffer.Thesectionsarethentypicallymountedon1%gel-coatedslidesandthenairdriedonaslidewarmer(at50degreesC)foratleastanhouruntilthroughlydriedandadheredtotheslide.Thesectionscanbestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.Themountedsectionswererehydratedindistilledwaterfor2minutesbeforebeingprocessedinthestainingsolutions. |
| Form | ThereagentsintheBlackGoldkitareallsuppliedinaliquidformatandareready-to-dilute. |
| Storage | Thekitcanbetransportedatroomtemperature.Oncereceived,thekitcanbestoredforupto12monthsat4°Cprotectedfromlight.Dilutedsolutionscanbestoreduptoonemonthat4°Cprotectedfromlight. |
| SpecificReferences | YingYLetal.(2014)Adultneuralprecursorcellsfromthesubventricularzonecontributesignificantlytooligodendrocyteregenerationandremyelination.JNeurosci.2014Oct15;34(42):14128-46 |
| KitComponents | Black-GoldII(Dilute1:10priortouse)-10mLSodiumThiosulfate,fixative(Dilute1:10priortouse)-10mLToluidineBlueO(Dilute1:10priortouse)-10mLAceticAcid(Dilute1:10priortouse)-10mL |
| ReagentKitprotocol |  TR-100-BG_April2012.pdf |
| MSDS | MSDS_TR-100-BG_BGT_as_at_Apr2014.pdf |
