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Biosensis/Black-Gold II Myelin Ready-to-Dilute Staining Kit with Congo Red Counter Stain for identifying Myelin & Amyloid Pla

价格
¥11940.00
货号:TR-200-BG
浏览量:127
品牌:Biosensis
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Description
Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
ReplacedbyTR-100-BG
OtherNamesblackandgold
ApplicationsBlackGoldIIisahighresolutionmyelinstainwithamyloidplaquecounterstain.Itsuseistailoredtostudiesusingformalinorparaformaldehydefixed,non-paraffinembedded,non-solventprocessedbraintissue.Itcanbeusedwithboththickandthinsections.Forthicksections,gelatincoatedslidesorslidesspeciallydesignedtobindtissuessectionsshouldbeusetoavoidsectionloss.Free-floatingsectionscanbeusedaswellbutsectionsareeasiertohandleandtransferwhenmountedonslides.Asuggestedmethodforthicksectionsisprovidedasaguide:Eitherfrozenorvibratomesectionsarecutatathicknessof20-50umandcollectedin0.1Mneutralphosphatebuffer.Thesectionsarethentypicallymountedon1%gel-coatedslidesandthenairdriedonaslidewarmer(at50degreesC)foratleastanhouruntilthroughlydriedandadheredtotheslide.Thesectionscanbestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.Themountedsectionswererehydratedindistilledwaterfor2minutesbeforebeingprocessedinthestainingsolutions.
FormThereagentsintheBlackGoldkitareallsuppliedinaliquidformatandareready-to-dilute.
StorageThekitcanbetransportedatroomtemperature.Oncereceived,thekitcanbestoredforupto12monthsat4°Cprotectedfromlight.Dilutedsolutionscanbestoreduptoonemonthat4°Cprotectedfromlight.
KitComponentsBlack-GoldII(Dilute1:10priortouse)-10mLSodiumThiosulfate,fixative(Dilute1:10priortouse)-10mLCongoRed(Dilute1:10priortouse)-10mLSodiumHydroxide(Dilute1:10priortouse)-10mL
ReagentKitprotocolClick to download the protocolTR-200-BG_April2012.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-200-BG_BGT_as_at_Apr2014.pdf