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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Amylo-Glo RTD Amyloid Plaque Stain Reagent with EtBr counter stain/TR-400-AG/1 Ea
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商品描述
| Description | TheBiosensisAG-400-AGkitutilizesanethidiumbromidecounterstainforaquickandeffectivewaytovisualizecellnucleiandcellbodiesofcellswhileunderUVIlluminationallowingtheassessmentofamyloidplaquesandcell/tissuepositioningaswellinonestep. Amylo-Glo®RTDReadytoDiluteStainingreagentisdesignedtostainamyloidplaquesintissuesections.ThisnovelMarkerhasseveraladvantagesoverotherconventionalmarkerssuchasThioflavinSandCongoRedbecauseofitsuniquechemicalandspectralproperties.(L.Schmuedetal.(2012)J.NeuroscienceMethods209:120126). UsingAmylo-Glo®resultsinaverybrightblueUVexcitablestainunderphysiologicalconditionsthatwillnotbleedthroughwhenilluminatedwithotherfilters.Itsbrightnessmakesitidealforlowmagnificationquantificationstudies,whileitsuniqueexcitation/emissionprofileandmildstainingconditionsmakesitidealforcombinationformultipleimmunofluorescentlabelingstudies.Amylo-Glo®RTDiscompatIBLewithfresh,frozen,andformalin-fixedimmunohistochemistryorcytochemistry,anditisparticularlygoodforconfocalandmultiplelabelingbecauseofitshighfluorescentintensityandhighresistancetophoto-bleaching.MoreoverbecauseAmylo-Glo®fluorescesintheUVchannel,doubleandtriplelabelingexperimentscanbeperformedveryeasily(seeprotocol). |
| Relatedproducts | TR-300-AG,MOAB-2antibody |
| BatchNo. | seeproductlabel |
| OtherNames | AmyloGlo |
| CompoundName | Compound:Amylo-Glo;Classification:Styrylbenzenederivative;Appearance:Yellowsolution;MolecularWeight:392;Filtersystemforvisualizing:UVEthibiumBromide:EtBr,2,7-Diamino-10-ethyl-6-phenylphenanthridiniumbromide;Appearance:lightred-orangesolution;MolecularWeight:394.32.392;Filtersystemforvisualizing:UV |
| Purity | Thinlayerchromatographyusingaluminaplatesandasolventsystemofethanolandwater(3:1)revealedthepresenceoftwofluorescentisomers.Noamountofstartingmaterialwasdetected. |
| Biol.activity | ExcitationPeakforAmylo-Glo:334EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.EtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm. |
| Applications | Stainingofamyloidplaquesinhumanandanimaltissues,seeincludedprotocol.EtBrcounterstainstainsnucleiandcellbodiesforeasyidentificationandspacialorientation. |
| Specificity | amyloidplaquesbothintraneuronalandvascularforA-G,Etbr,nucleiandcellbodiesbothDNAandRNAlabel |
| Appearance | ExcitationPeak:334;EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.TovisualizeAmylo-glointissue,UVlightisrequired.Forexample,Amylo-GlotissuecanbeexaminedusinganepifluoresentmicroscopewithUV(NikonUV-2A)filtercube.Excitation(325-375nm)Emission(400-450nm)istypical.Alsonote,itisnotuncommonforAmylo-Glotoappearlightyellowwhenexaminedbyeye,yetappearalightbluecolorwhenphotographed. VisualizationofEtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm.MostUVcompatiblefiltersetscanbeused. |
| Reconstitution | Readytodiluteperprotocol10Xsolutions |
| Storage | Thestocksolutioncanbestoredforupto6monthsat4°Cprotectedfromlight.Nopreservatives.Usesteriletechniquewhenhandlingandproperlaboratoryprocedures. |
| ExpiryDate | 6monthsfromdateofpurchase |
| GeneralReferences | L.Schmuedetal.(2012)J.NeuroscienceMethods209:120126 |
| KitComponents | 1bottlecontaining40mlof10XAmylo-Glo®RTD(A-GRTD)solution1bottlecontaining40mlof10XA-GRTDEthidiumBromide(EtBrRTD)solution |
| ReagentKitprotocol |  TR-400-AG_as_at_Apr2014_protocol.pdf |
| MSDS | MSDS_TR-400-AG_as_at_July2015.pdf |
