品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer/B-100-PS/100 mL
商品描述
| Description | Detergentstypicallypresentincelllysisbuffersarethoughttodisruptorganellesandcompartmentsandincreasetheexposureofsolublephospho-proteinstophosphatasesandproteasestherebyresultinginuncontrolleddephosphorylationandproteolysis. Phospho-SureRTDNeuronalextractionbufferisoptimizedfortheextractionofphosphorylatedproteinsfromneuronalandothersofttissuestypes.Thebufferextractsthephosphoproteinsinanativestatewithouttheuseofharshdetergentsoroxidizers,anditisspeciallyformulatedtohelpmaintainphosphoproteinsandprotectthemfromdegradationbetterthantrADItionaldetergentbasedextractionbuffers. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100mL |
| OtherNames | Phosphosure |
| Applications | PleasedownloadtheprotocolbelowfordetailedinstructionsonhowtousePhospho-Sureinneuronalandothersofttissues. |
| FeaturedArticles | RefertothepublicationbySunejaetal(2006)wherethisbufferwasshowntoimproverecoveryofphospho-proteinssuchasERK1/2-PandSAP/JNK-Pinthedissectionandhomogenizationofguineapigbraintissue. |
| Comments | Phospho-SureRTDisdesignedasbothanisolationandextractionbufferandforbestresultstheintacttissueshouldbebathedinice-coldPhospho-surebufferduringexcisionofthetissue.OncethedesiredtissuepieceisisolatedinPhospho-sureRTDitmaybetransferredtoasmallvolumeofice-coldPhospho-SureRTDfordisruption. |
| Form | Powdercontainingnopreservatives.PreparationofPhospho-SureRTDisverysimple.SimplyaddsterileddH2Odirectlytothepowderedsolutioninthesuppliedbottle,swirlbrieflytodissolvethechemicalsanduse.Thematerialcontainsnopreservativessosteriletechniqueshouldalwaysbeused. |
| Appearance | Whitepowder |
| Reconstitution | Add93mlofsteriledoubledistilledwaterandmixbygentlyswirling,finalvolume100mlat20°C. |
| Storage | Thedry,unopenedcontainershouldbestoredatroomtemperatureinadryordesiccatedlocationprotectedfromlight.Donotstoreintherefrigeratorunlessmaterialisinadry,moisturefreeenvironment.Materialishydroscopicsooncethesealisbrokenitshouldbehydratedandnotresealedwhiledry.Oncehydrated,thebuffercanbestoredat4°Cforupto3months.Solutioncanbefrozenbutclumpingmayoccuruponthawingandisnotrecommended. |
| ExpiryDate | Powderedformatcanbestoredupto12monthsafterpurchase. |
| SpecificReferences | 1.SunejaSK,MoZ,PotashnerSJ.(2006)Phospho-CREBandotherphospho-proteins:improvedrecoveryfrombraintissue.JNeurosciMethods.2006Jan30;150(2):238-41. 2.ElviraMass,DagmarWachten,AnnaC.Aschenbrenner,AndréVoelzmann,MichaelHochemail(2014)MurineCreld1ControlsCardiacDevelopmentthroughActivationofCalcineurin/NFATc1Signaling,DevelopmentalCellVolume28,Issue6,p711726 |
| Bufferprotocol |  B-100-PS_as_at_March2013.pdf |
| MSDS | MSDS_B-100_B-200_as_at_Apr2014.pdf |
