The LEGENDplex™ Mouse B cell Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel focuses on 13 key biomarkers involved in B cell function, activation, and survival. It allows for simultaneous quantification of 13 mouse targets such as IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, and IL-10. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
The LEGENDplex™ Mouse B cell Panel is configured as shown below depending on sample types and required dilutions:
Catalog No. | Plex Size | Targets | Recommended Sample Type | Recommended Dilution Factor |
740818 740819 | 13-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, IL-10 | Tissue Culture* | Varies |
740826 740827 | 12-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BCMA, sCD40L, IL-10 | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740982 740983 | 1-plex | BAFF | Serum, Plasma | 25 |
Tissue Culture | Varies | |||
740824 740825 | 3-plex | IL-12p70, TNF-α, IFN-γ | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740822 740823 | 5-plex | IL-4, IL-6, IL-2, TNF-α, IL-13 | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740820 740821 | 8-plex | IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, IL-13, IFN-γ | Serum, Plasma | 2 |
Tissue Culture | Varies | |||
740828 740829 | 2-plex | Free Active TGF-β1, IL-10 | Serum, Plasma | 2 |
Tissue Culture | Varies |
*Note: Serum and plasma are not the recommended sample type. Refer to Sample Dilution section in the manual for details.
The LEGENDplex™ Mouse B cell Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel. This assay is for research use only.
Product Details Biolegend揭示出对神经系统以及影响神经系统的疾病的更好理解的研究可以转化为改善数百万生命的治疗方法。BioLegend提供广泛的产品组合，为您的神经科学实验提供解决方案，包括抗体，免疫测定溶液，重组蛋白，磁性细胞分离和单细胞蛋白质组学试剂。我们的试剂是与该领域的杰出领导者（如迈克尔·J·福克斯基金会）合作建造的（请参见下面的“产品亮点”标签），这表明我们承诺与您的团队合作寻找新发现，为我们的客户带来新的选择和全面的解决方案研究神经系统疾病的解决方案。
