Description

Go-ChIP-Grade™ProteinGEnzymaticKitprovidesasensitive,reliable,andefficientmethodforenzymaticChromatinIP.ThekitiscompletewithallreagentsandbuffersnecessaryforChIPassayanditrevolutionizestheuseofspincolumnsforChIPassay.Eachcolumncontainsadiscofaninert,porouspolymertowhichproteinGhasbeencovalentlyattached.Duringtheassay,thetargetchromatin/antibodycomplexisretainedbythedisc.FlushingwiththreewashbuffersandanelutionstepareallthatisrequiredtoobtaintheDNAfragmentsofinterest. Go-ChIP-Grade™ProteinGEnzymaticKitispreferabletoothermethodsbecause:

• Go-ChIP-Grade™ProteinGEnzymaticKitcolumnseliminatetheneedsforbeads.
• Go-ChIP-Grade™ProteinGEnzymaticKitcolumnscontainaproprietaryporouspolymerdisctowhichproteinGhasbeencovalentlyanchored.Itsporeshaveinnersurfacesspecificallydesignedtomaximizechromatincaptureefficiency.
• Thediscbasematerialischemicallyinert,reducingincidencesofnon-specificbinding.
• Allchromatincapturetakesplaceinsidethedisc,thereforethereisnodependenceonriskyPipetteseparationprocedures.
• Thestandardprotocoldoesnotrequirepre-blockingandpre-washsteps.
• Flushingawayunboundchromatinandotherunwantedmaterialiseasilyachievedbyusingwashingstepsthatarefastandlesspronetoerror. 

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