DESCRIPTION:

A fully validated, highly specific rabbit polyclonal antibody to Serine-38 phosphorylated forms of SERCA2

SERCA2 belongs to the P-type family of ATPases (Xuet al., 1993; Toyofukuet al., 1994; Hawkinset al., 1994; Osadaet al., 1998; Netticadanet al., 1999; Netticadanet al., 2000). Controversy still surrounds the phosphorylation status of Ser-38 and its physiological implication. Some groups have reported that Ser-38 of SERCA2 is phosphorylated by CaMKII, which apparently leads to a substantial increase in ATPase activity (Xuet al., 1993; Hawkinset al., 1994). However, the control of Ca²⁺pump function by direct phosphorylation has not been observed by all investigators (Odermattet al., 1996; Reddyet al., 1996; Rodriguezet al., 2004). Independent attempts to confirm SERCA2 phosphorylation at Ser-38 have failed to confirm the stimulation of Ca²⁺pump function upon treatment with CaMKII, which suggests that Ser-38 phosphorylation of SERCA2 is not a significant regulatory feature of cardiac Ca²⁺homeostasis. This antibody, described by Rodriguezet al. (2004), may help to resolve the controversy, as it recognises the Ser-38 phospho-epitope

Tested applications:Western Blot(1:1000 dilution).Species recognition:Epitope sequence exists in SERCA2 from all mammalian species.

DATA SHEET:

Description	Lyophilised rabbit polyclonal affinity purified antibody containing IgG antibody specific for Ser-38 phosphorylated forms of SERCA2 (Rodriguez et al., 2004)
Immunogen	Synthetic peptide (K31LKERWGS(PO3H2)NEL41) corresponding to amino acids surrounding the phosphorylated serine residue at position 38 of SERCA2, conjugated to KLH.
Isotype	IgG
Purification	Protein A affinity purified
Species reactivity	The antibody recognises the phospho Ser-38 epitope within a peptide, which is part of a positive control protein since Ser-38 has yet to be shown to be phosphorylated in canine cardiac SR samples (Rodriguez et al., 2004)
Tested applications	WB, ELISA
Recommended dilution	WB 1:1000
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4°C. Reconstituted:-80°C
Datasheet	A010-25AP
MSDS	A010-25AP
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.

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