DESCRIPTION:
Afullyvalidated,highlyspecificmousemonoclonalantibodytocMyc
c-Myc,isapolypeptidetagofsequence(NH3-EQKLISEEDL-COOH)derivedfromtheregulatorproteinmyc.CommonlyintegratedintorecombinantproteinsasanNorCterminaltag,theMyctagisusedtofacilitateaffinitychromatography,proteinlocalisationorprotein-proteininteractionstudies.TheBadrillaMycmonoclonalantibodyfromclone9E10.2producesexceptionalstaininginbothimmunoblottingandimmunohistochemicalapplicationsandcanbeusedatextremelyhighdilutions.
Testedapplications:Westernblot(1:20,000dilution),IFMicroscopy(1:1000dilution).Antibodyform:LyophilisedAffinityPurifiedIgG1.Speciesrecognition:HumancMyc
DATASHEET:
Description | Lyophilisedmousemonoclonalantibodyclone9E10.2 |
Immunogen | SyntheticpeptideEQKLISEEDLderivedusingthesequenceofhumanc-mycgeneproduct |
Isotype | IgG |
Purification | ProteinGaffinitypurified |
Speciesreactivity | Doesnotreactwithmouseorchickenc-myc |
Testedapplications | WB,IF |
Recommendeddilution | WB40pg/ml(1:20,000dilution),IF0.8ng/ml(1:1000dilution) |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin60µlof18MΩH2Otoproducea0.8µg/mlsolution.Aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°CReconstituted:-80°C |
Datasheet | A010-Myc-50 |
MSDS | A010-Myc-50 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |