Description:
ReadyFect is a powerful, lipid-based transfection reagent based on Triggered Endosomal Escape technology that has been optimized for use with Axol Neural Stem Cells. ReadyFect provides a simple, quick and convenient transfection method that has minimal toxicity without impacting differentiation potential.
Optimized Lipid Based Transfection of Human Neural Stem Cells
ReadyFect is a powerful, lipid-based transfection reagent based on Triggered Endosomal Escape technology that has been optimized for use with our Human Neural Stem Cells. ReadyFect provides a simple, quick and convenient transfection method that has minimal toxicity without impacting differentiation and phenotype potential.
Optimizing Lipid-Based Transfection of Neural Stem Cells
9 lipids (NL-1 to NL-9) were tested using 3 ratios: pVectOZ-GFP encoding for GFP protein was used and fluorescence intensity was measured 2 days after transfection. ReadyFect (NL-7) gave the best fluorescence intensity of the Lipid-Based Transfection Reagents tested.
Protocol Overview
An overview of the protocol for transfecting Human Neural Stem Cells using ReadyFect.
Product Specification:
Size | 1 x 40 μL vial |
Shipping conditions | Blue ice |
Storage conditions | -20°C |
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