Protein PurificationBaculovirus ExpressionE. coli ExpressionMolecular BiologyProducts Products: Kinases: PAK7KD PAK7KD (recombinant human PAK7 kinase domain, aka PAK5) Catalog #: Size: Concentration: Price: K0108S 10 µg 1 mg/ml $375 K0108M 100 µg 1 mg/ml $1,375 K0108L 1 mg 1 mg/ml $5,640 K0108B Bulk 1 mg/ml Inquiry To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490 PRODUCT DETAIL DATA SHEET Sequence: GPHPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVI MRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDS ILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQA IRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH The first 4 residues GPHP are from Turbo3C cleavage site. Phosphorylated at S602. Component and Formulation: PAK7KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT. Activity: Specific activity:4,199 pmoles/min/µg Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen): Different concentrations of PAK7KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature. Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters. SDS-PAGE Analysis: Protein Purification |Baculovirus Expression | Mammalian Expression |E. coli Expression |Molecular Biology | Proteins tel: 858-678-8618fax: 888-239-6490 toll-free: 888-365-8085email: info@accelagen.comCopyright © 2020 Accelagen Inc.All rights reserved.

苏州蚂蚁淘生物技术研究所(ebiomall)销售Accelagen产品，美国Accelagen蛋白激酶/高纯蛋白/酶工具 Accelagen公司迅速的从基因研究到蛋白质研究成长起来的一个公司,它可以提供gene-to-protein全方位解决方案。Accelagen公司生产的重组蛋白已经广泛应用于基于结构的药物设计（SBDD）和高通量筛选（HTS）领域，用于各大药厂的药物研究与生产。其中Turbo3C （HRV3C）Protease、TurboTEV Protease、TurboNuclease是Accelagen公司极具性价比的优质产品。Accelagen重组蛋白表达和纯化服务可为生物学科研人员及药物研究提供高品质的重组蛋白。 H0101B Turbo3C (HRV3C) Protease Bulk 请询价   Accelagen   H0101M Turbo3C (HRV3C) Protease 10mg (10,000 Units) ￥25200   Accelagen   H0101S Turbo3C (HRV3C) Protease 1mg (1,000 Units) ￥2800   Accelagen   N0103B TurboNuclease Bulk 请询价   Accelagen   N0103L TurboNuclease 100,000 Units ￥4900   Accelagen   N0103M TurboNuclease 50,000 Units ￥2520   Accelagen   T0102B TurboTEV Protease Bulk 请询价   Accelagen   T0102L TurboTEV Protease 10mg (100,000 Units) ￥37800   Accelagen   T0102M TurboTEV Protease 1mg (10,000 Units) ￥4200   Accelagen