UniProt Accession Number of Target Protein: Q3U0X8

Alternative Name(s) of Target: TACTILE; T-cell surface protein tactile; T cell-activated increased late expression proteinImmunogen: This antibody was raised by immunising rats with murine CD96.

Specificity: This antibody is specific for murine CD96, and recognises an epitope in the first Ig domain.

Application Notes: This antibody blocks murine CD96-CD155 interaction, and has been shown to bind to the first Ig domain of CD96 and compete with CD155 binding (Roman Aguilera et al, 2018). This antibody displays significant anti-metastatic activity in four experimental lung metastases models and one spontaneous tumour metastasis model (Roman Aguilera et al, 2018), with a potency greater than the anti-CD96 monoclonal antibodies 3.3 and 8B10. The binding specificity of this antibody has been determined in flow cytometric analysis of HEK-293 cells transfected with a range of CD96 Ig domain chimeric constructs (Roman Aguilera et al, 2018).

Antibody first published in:Seth et al.The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1.Biochem Biophys Res Commun. 2007 Dec 28;364(4):959-65.PMID:17971293Note on publication:Describes the original generation of this antibody, and its characterisation.