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蚂蚁淘在线 / 品牌中心 / Absolute Antibody / absoluteantibody/Ab00806-1.6 Anti-HIV-1/HIV-2 protease [1696]/Ab00806-1.6/ 100 µg
商品描述
Description:RecombinantmonoclonalantibodytoHIV-1/HIV-2protease.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridoma1696.ThisisaFabfragmentwithahis-tag.
CloneNumber:1696
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):Neutralize;ELISA;WB
PublishedSpeciesReactivity:HIV-1;HIV-2
Immunogen:1696waspreparedbygeneratingahybridomacellline,fusingmyelomacellswithsplenocytesderivedfromBALB/cmiceimmunizedwithHIV-1PRinthepresenceofFreund"sadjuvant.
Specificity:1696bindstoHIV-PRofbothHIV-1andHIV-2(IgG1696:HIV-1PRpH5.7~350uM;pH7.4~100nM-HIV-2PRpH5.7nomeasurableinteraction;pH7.4~30nM--Fab1696:HIV-1PRpH5.7~410nM;pH7.4~130nM-HIV-2PRpH5.7nomeasureableinteraction;pH7.4~40nM--determinedbyELISA).TheantibodybindstotheN-terminalregionoftheenzyme,andbindingisinhibitedbythepeptidefragmentPQITLWQwhichrepresentsresidues1-7.Westernblotbindingassaysshowthat1696recognizesthematureandprocessedformoftheenzyme,butnottheprecursorwhichpossesses20residuesupstreamfromthecleavagesite.TheN-terminushasawellconservedstructurebetweenHIV-1PRandHIV-2PR.HIV-PRisahomodimericapartateproteasethatspecificallycleavestheviralGagandGag/Polpolyproteinprecursor.Acorrelationhasbeenobservedbetweentheactivityoftheproteaseandthedegreeofinfectivity,andsothisproteinisamajorantiviraltarget.
ApplicationNotes:1696mAbcaninhibitthecatalyticactivityofbothHIV-1andHIV-2HIV-PR(1696IgGHIV-1PRIC50~0.6nM;HIV-2PRIC50~1.5nM--1696FabHIV-1PRIC50~1.1nM;HIV-2PRIC50~2.6nM).Inhibitionby1696ismediatedbythedestABIlizationoftheoftheactiveformoftheactiveHIV-PRhomodimerthroughbindingtotheN-terminus,whichconstitutesalargepercentageoftheinterfacebetweenthemonomers.TheFabfragmentwasgeneratedbyincubationoftheantibodywithpepsin.Bindingconstantsof1696canbedeterminedbyELISA.1696showsreactivitytothematureprocessedHIV-PRandnottotheprecursorbyWB.
AntibodyFirstPublishedin:
Lescaretal.InhibitionoftheHIV-1andHIV-2proteasesbyamonoclonalantibodyProteinSci.1999Dec;8(12):2686–2696.Noteonpublication:DescribesthegenerationofthemAb1696,itsfunctionalcharacterizationandthedeterminationofthecrystalstructureoftheFabfragment.
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals. Absolute Antibody我们现在与Digital Proteomics合作提供多克隆永生化服务。这意味着您可以生产可复制且不受限制的多克隆试剂，或者利用天然免疫系统来识别新的抗体候选物。对于这项服务，您可以提供免疫宿主生物的血清和B细胞，纯化的抗原用于免疫，或者只是告诉我们您的靶标，我们将做剩下的事情。鉴于其中之一，我们可以提供多达10条由发现引擎选择的重链序列和10条轻链序列，在交互式报告中提供具有蛋白质组学证据的扩展库，根据要求将抗体工程化为任何种类和同种型，以及我们提供的抗体表征数据获得。此服务是100％免版税的。 ThisreformattedmouseantibodywasmadeusingthevariabledomainsequencesoftheoriginalMouseIgGformat,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:MouseFabfragment,His-Tagged,kappaCloneNumber:1696
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):Neutralize;ELISA;WB
PublishedSpeciesReactivity:HIV-1;HIV-2
Immunogen:1696waspreparedbygeneratingahybridomacellline,fusingmyelomacellswithsplenocytesderivedfromBALB/cmiceimmunizedwithHIV-1PRinthepresenceofFreund"sadjuvant.
Specificity:1696bindstoHIV-PRofbothHIV-1andHIV-2(IgG1696:HIV-1PRpH5.7~350uM;pH7.4~100nM-HIV-2PRpH5.7nomeasurableinteraction;pH7.4~30nM--Fab1696:HIV-1PRpH5.7~410nM;pH7.4~130nM-HIV-2PRpH5.7nomeasureableinteraction;pH7.4~40nM--determinedbyELISA).TheantibodybindstotheN-terminalregionoftheenzyme,andbindingisinhibitedbythepeptidefragmentPQITLWQwhichrepresentsresidues1-7.Westernblotbindingassaysshowthat1696recognizesthematureandprocessedformoftheenzyme,butnottheprecursorwhichpossesses20residuesupstreamfromthecleavagesite.TheN-terminushasawellconservedstructurebetweenHIV-1PRandHIV-2PR.HIV-PRisahomodimericapartateproteasethatspecificallycleavestheviralGagandGag/Polpolyproteinprecursor.Acorrelationhasbeenobservedbetweentheactivityoftheproteaseandthedegreeofinfectivity,andsothisproteinisamajorantiviraltarget.
ApplicationNotes:1696mAbcaninhibitthecatalyticactivityofbothHIV-1andHIV-2HIV-PR(1696IgGHIV-1PRIC50~0.6nM;HIV-2PRIC50~1.5nM--1696FabHIV-1PRIC50~1.1nM;HIV-2PRIC50~2.6nM).Inhibitionby1696ismediatedbythedestABIlizationoftheoftheactiveformoftheactiveHIV-PRhomodimerthroughbindingtotheN-terminus,whichconstitutesalargepercentageoftheinterfacebetweenthemonomers.TheFabfragmentwasgeneratedbyincubationoftheantibodywithpepsin.Bindingconstantsof1696canbedeterminedbyELISA.1696showsreactivitytothematureprocessedHIV-PRandnottotheprecursorbyWB.
AntibodyFirstPublishedin:
Lescaretal.InhibitionoftheHIV-1andHIV-2proteasesbyamonoclonalantibodyProteinSci.1999Dec;8(12):2686–2696.Noteonpublication:DescribesthegenerationofthemAb1696,itsfunctionalcharacterizationandthedeterminationofthecrystalstructureoftheFabfragment.
ProductForm
Purification:PurifiedbyImmobilizedMetalAffinityChromatographySuppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
