品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

AAT Bioquest/Amplite™ Fluorimetric Calcium Quantitation Kit *Red Fluorescence*/36360/200 Tests

价格
¥56560.00
货号:36360
浏览量:127
品牌:AAT Bioquest
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Overview
PrinterFriendlyVersion

Ex/Em(nm)551/577
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
MetalIons
RelatedDiagnosticMolecules
BiochemicalAssays
Calciumisessentialforalllivingorganisms,particularlyincellphysiology,wheremovementofthecalciumionCa2+intoandoutofthecytoplasmfunctionsasasignalformanycellularprocesses.Calciumisthefifthmostabundantelementbymassinthehumanbody,whereitisacommoncellularionicmessengerwithmanyfunctions,andservesalsoasastructuralelementinbone.Calciumplaysanimportantroleinmediatingtheconstrictionandrelaxationofbloodvessels,nerveimpulsetransmission,musclecontraction,andhormonesecretion.Theserumlevelofcalciumiscloselyregulatedwithinafairlylimitedrange(9to10.5mg/dL)inthehumanbody.Bothhypocalcaemiaandhypercalcaemiaareseriousmedicaldisorders.Causesoflowcalciumlevelsincludechronickidneyfailure,vitaminDdeficiency,andlowbloodmagnesiumlevelsthatcanoccurinseverealcoholism.Amplite™CalciumDetectionKitusingourproprietaryredfluorescenceprobeprovidesasimplemethodfordetectingcalciuminphysiologysolutions.ThefluorescentsignalcanbeeasilyreadbyfluorescencemicroplatereaderwithEx/Em=540/590nm.TheAmplite™CalciumDetectionKitcanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithnoseparationstepsrequired.Theassaycanbecompletedwithin30minutes.WiththeAmplite™CalciumDetectionKit,wehavedetectedaslittleas0.03mMcalcium.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note:Thawallthekitcomponentstoroomtemperaturebeforestartingtheexperiment.


 

1.Preparestocksolutions:

Prepare200XRhodRed™stocksolutionbyadding50µLofsterileH2OintothevialofRhodRed™Indicator(ComponentA).Thestocksolutionshouldbeusedpromptly.Anyremainingsolutionneedstobealiquotedandrefrozenat-20oC.

 

2.Prepareassayreactionmixture:

Prepareassayreactionmixtureaccordingtothefollowingtable,keptfromlight.

 

Table1.Assayreactionmixtureforone96-wellplate

Components

Volume

RhodRed™stocksolution(200X,fromStep1)

25μL

AssayBuffer(ComponentB)

5mL

TotalVolume

5.025mL

 

Table2.Layoutofcalciumstandardsandtestsamplesinasolidblack96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

CS1

CS1

….

….

….

….

 

 

 

 

 

 

CS2

CS2

 

 

 

 

 

 

 

 

 

 

CS3

CS3

 

 

 

 

 

 

 

 

 

 

CS4

CS4

 

 

 

 

 

 

 

 

 

 

CS5

CS5

 

 

 

 

 

 

 

 

 

 

CS6

CS6

 

 

 

 

 

 

 

 

 

 

CS7

CS7

 

 

 

 

 

 

 

 

 

 

              Note:CS=CalciumStandards,BL=BlankControl,TS=TestSamples.

 

Table3.Reagentcompositionforeachwell

CalciumStandard

BlankControl

TestSample

Serialdilutions*:50μL

H2O:50μL

50μL

*Note:Addtheseriallydilutedcalciumstandardsfrom3mMto0.003mMintowellsfromCS1toCS7induplicate.

 

3.RunCalciumassay:

3.1   Prepareacalciumstandardbydilutingtheappropriateamountofthe300mMCalciumStandard(ComponentC)intoH2OtoproduceaCalciumconcentrationrangingfrom0to3mM(12mg/dL).A0 mMcalciumcontrolisincludedasblankcontrol.Thefinalcalciumconcentrationswillbetwofoldslower(i.e.,0to1.5mM)withtheadditionofassayreactionmixture(SeeStep3.3).

 

3.2   Add50μLofserialdilutedcalciumstandard(fromStep3.1)intoeachwell.

 

3.3   Add50μLofassayreactionmixture(fromStep2,Table1)toeachwellofcalciumstandard,blankcontrol,andtestsamples(seeStep2,Table3)tomakethetotalcalciumassayvolumeof100µL/well.

Note:Fora384-wellplate,add25μLofsampleand25μLofassayreactionmixtureintoeachwell.

 

3.4   Incubatethereactionfor5to30minutesatroomtemperature,protectedfromlight.

 

3.5   MonitorthefluorescenceintensitywithafluorescenceplatereaderatEx/Em=540/590nm.

References&Citations
CitationExplorer

Highefficiencysingle-stepBiomaterial-basedmicroparticlefabricationviatemplate-directedsupramolecularcoordinationchemistry
Authors:KwokKeiLai,ReinhardRenNEBerg,WingCheungMak
Journal:GreenChemistry(2016):1715--1723

Microcystin-LRcausessexualhormonedisturbanceinmaleratbytargetinggonadotropin-releasinghormoneneurons
Authors:XuetingWang,JieDing,ZouXiang,PeipeiJiang,JingDu,XiaodongHan
Journal:Toxicon(2016):45--55


美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。AAT Bioquest会不断介绍新产品,快速的丰富各个领域的产品。作为AAT Bioquest Inc.的中国区域代理,蚂蚁淘科技为中国客户提供光谱学检测领域,包括底物显色、荧光和发光技术等全系列解决方案。       1、更具品质保障的AAT Bioquest产品(AAT Bioquest作为全球专业的光谱学检测产品生产商,能提供最具性价比的产品); 2、更高价格优势和性价比的选择,我们将一如既往的为客户和代理商提供最具价格竞争力的产品和全面的售后保障(作为全国总代理,我们能提供最大的价格优惠和更全面的技术支持) 3、更快捷的到货时间,我们将以更短的订货周期,更快捷的国际物流以及报关时间为您提供最节省时间的供货保证(蚂蚁淘具有独立的进出口和报关权,因此提供更快捷的到货保障);