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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
HostSpecies:Mouse
Clonality:cloneHA.C5/C10
Isotype:IgG3
Background:
Hemagglutininisanantigenicglycoproteinfoundonthesurfaceoftheinfluenzaviruses,aswellasmanyotherbacteriaandviruses.ItisresponsIBLeforbindingthevirustothecellthatisbeinginfected.Thenamehemagglutinincomesfromtheprotein"sABIlitytocauseredbloodcells(erythrocytes)toagglutinateinvitro.TheHAtagisderivedfromtheHAmoleculecorrespondingtoa.a.98-106hasbeenextensivelyusedasageneralepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.Thistagfacilitatesthedetection,isolation,andpurificationoftheproteins.
Description:
Affinitypurifiedanti-HA-tagcloneHA.C5/C10,mouseIgG3.RecognizestheN-terminal,C-terminalorinternalHA-taggedfusionproteins.Applications:WesternBlot(1:1000),DotBlot,ELISA,Immunocytochemistry(1:200),Immunoprecipitation(1:200),InSituHybridisation(1:500-2000).Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
Synonym(s):
Antibody,HA-tag
Formulation:
1mg/mlin10mMPBS,pH7.2,with0.05%NaN3
SpeciesReactivity:
RecognizestheHemagluttininepitopetaginrecombinantproteins
CrossReactivity:
Worksinhumancells;predictedtoworkinallproteinscontainingtheHAtag,regardlessofspecies.
Purification:
Affinitychromatography
Immunogen:
Syntheticpeptidefrominfluenzahemagglutininepitope:YPYDVPDYAconjugatedtoKLH.
Format:
Aqueousbuffersolution
Storage/Stability:
Storeat–20°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat+4°Cfor1-2weeks.
Application(s):
WB,DB,ELISA,IP,ICC
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Antibodies
ProductType:
Antibody
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