品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
HostSpecies:Mouse
Clonality:cloneGF28R
Isotype:IgG1
Background:
Greenfluorescentprotein(GFP)isa27kDaproteinderivedfromthejellyfishAequoreavictoria,whichemitsgreenlight(emissionpeakatawavelengthof509nm)whenexcitedbybluelight(excitationpeakatawavelengthof395nm).GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.GFPfluorescenceisstableunderfixationconditionsandsuitableforavarietyofapplications.GFPhasbeenwidelyusedasareporterforgeneexpression,enablingresearcherstovisualizeandlocalizeGFP-taggedproteinswithinlivingcellswithouttheneedforchemicalstaining.OtherapplicationsofGFPincludeassessmentofproteinproteininteractionsthroughtheyeasttwohybridsystemandmeasurementofdistancebetweenproteinsthroughfluorescenceenergytransfer(FRET)protocols.GFPtechnologyhasconsiderablycontributedtoagreaterunderstandingofcellularphysiology.YFPdiffersfromGFPduetoamutationatT203Y;antibodiesraisedagainstfull-lengthGFPshouldalsodetectYFPandothervariants.
Description:
AffinitypurifiedAnti-GFP(GreenFluorescentProtein)TagcloneGF28R,mouseIgG1.RecognizesnativeanddenaturedformsofGFPanditsvariantssuchas:EGFP,YFP,EYFP,andCFP.Applications:WesternBlot(1:1000-3000),DotBlot,ELISA,Immunocytochemistry(1:500-2000),Immunoprecipitation.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
Synonym(s):
Antibody,GFP-tag
Formulation:
1mg/mlin10mMPBS,pH7.2,with0.05%NaN3
SpeciesReactivity:
Aequoreavictoria.
CrossReactivity:
RecognizesnativeanddenaturedformsofjellyfishGFPanditsvariantssuchas:EGFP,YFP,EYFP,andCFP.Doesnotreactwithcoral-derivedfluorescentproteins.
Immunogen:
SyntheticpeptideconjugatedtoKLH,correspondingtoN-terminala.a.ofGFPfromthejellyfishAequoreavictoria.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat–20°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat+4°Cfor1-2weeks.
Application(s):
WB,DB,ELISA,IP,ICC
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
