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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:69.5kDa
Genbank#:AF245447
Tag(s):N-terminalHis-tag
a.a:fulllength
Description:
HumanSphingosinekinase2(GenBankAccessionNo.AF245447),fulllengthwithN-terminalHis-tag,MW=69.5kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
SphK2,sphingosinekinase2,SPK2
SpecificActivity:
20U/µg
UnitDefinition:
Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1Pperminuteat37°C.
AssayConditions:
SphingosinekinaseactivitywasdeterminedusingD-erythro-sphingosineandATPassubstrates.Assaycondition:50mMHEPES,pH7.4,150mMNaCl,5mMMgCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP,4µMD-erythrosphingosine,and0.75μg/mlsphingosinekinase2at37°Cfor30min.ATPreductionisdetectedusingKinase-Glo®LuminescentKinaseAssayPlatform(PromegaCorporation,MADIson,WI).
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,225mMimidazole,20%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Zemann,B.,etal.,Blood.107(4):1454-8(2006)
2.Maceyka,M.,etal.,JBiolChem.280(44):37118-29(2005)
ApplicationReference:
1.SynthesisandBIOLOGicalevaluationofcarbocyclicanaloguesofpachastrissamine(2015)
2.RegulationofthehepatitisCvirusRNAreplicasebyendogenouslipidperoxidation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
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