品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:74kDa
Genbank#:NM_020126
Tag(s):N-terminalHis-tag
a.a:fulllength
Description:
HumanSphingosinekinase2(GenBankAccessionNo.NM_020126),fulllengthwithN-terminalHis-tag,MW=74kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem
UniProt
P08571
Synonym(s):
SphK2,sphingosinekinase2,SPK2
SpecificActivity:
20U/µg
UnitDefinition:
Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1Pperminuteat37°C.
AssayConditions:
SphingosinekinaseactivitywasdeterminedusingD-erythro-sphingosineandATPassubstrates.Assaycondition:50mMHEPES,pH7.4,150mMNaCl,5mMMgCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP,4µMD-erythrosphingosine,and0.75μg/mlsphingosinekinase2at37°Cfor30min.ATPreductionisdetectedusingKinase-Glo®LuminescentKinaseAssayPlatform(PromegaCorporation,MADIson,WI).
Formulation:
25mMTris-HCl,pH8.0,100mMNaCl,0.05%Tween-20,50%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
Okada,etal.,J.Biol.Chem.280:36318-36325(2005).
ApplicationReference:
RegulationofthehepatitisCvirusRNAreplicasebyendogenouslipidperoxidation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
