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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:46.9kDa
Genbank#:NM_001142601.1
Tag(s):N-terminalHis-tag
a.a:fulllength
Description:
HumanSphingosinekinase1orSphK1(GenBankAccessionNo.AF200328),fulllengthwithN-terminalHis-tag,MW=46.9kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q9NYA1-1
Synonym(s):
SphK1,sphingosinekinase1,SPK1
SpecificActivity:
3,300pmole/min/μg
UnitDefinition:
Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1Pperminuteat37°C.
AssayConditions:
AssaywasdoneinKinasebuffercontaining20μMSphingosineand20μMATP.Reactionwasdoneat30°Cfor35min.AmountofATPtransferredwascalculatedusingAssaywasdoneinKinasebuffer(40mMTris,pH7.4,20mMMgCl2+,0.1mg/mlBSA)containing20μMSphingosineand20μMATP.Reactionwasdoneat30°Cfor35min.AmountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega)
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,10%glycerol,225mMimidazole,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Delon,C.,etal.,J.Biol.Chem279(43)44763-44774(2004).
2.Taha,TA.,etal.,FASEBJ.20(3):482-4(2005).
ApplicationReference:
RegulationofthehepatitisCvirusRNAreplicasebyendogenouslipidperoxidation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
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