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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:209kDa(complex)
Genbank#:U79143(p110α),XM_043865(p85α)
Tag(s):N-terminalFLAG-tag(p110α),notagonp85α
a.a:fulllength
Description:
ComplexofN-terminalFLAG-taggedrecombinantfull-lengthhumanp110α(GenBankAccessionNo.U79143),withE545Kmutation,andrecombinantfulllength,humanp85α(notag,GenBankAccessionNo.XM_043865).Co-expressedinSf9cellsinaBaculovirusexpressionsystem.p110α(E545K)MW=125kDa,p85αMW=84kDa.
Synonym(s):
PIK3CA,p110alpha,Phosphoinositide-3-Kinase,Catalytic,Alpha,PIK3R1,Phosphoinositide-3-Kinase,RegulatorySubunit1(Alpha),GRB1,p85alpha
SpecificActivity:
800 pmol/min/μg
UnitDefinition:
Aunitofp110α(E545K)/p85αkinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofPhosphoinositol-3,4,5-Trisphosphate/minat37°C.
Formulation:
25mMTris-HCl,pH8.0,69mMNaCl,1.35mMKCl,0.03%Tween-20,3mMDTT,50%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Engelman,J.A.,etal.NatureMed.14:1351-1356(2008)
2.Hafner,C.,etal.,Proc.Natl.Acad.Sci.USA.104(33):13450-13454(2007)
3.Serra,V.,etal.,CancerRes.68(19):8022-8030(2008)
2.Hafner,C.,etal.,Proc.Natl.Acad.Sci.USA.104(33):13450-13454(2007)
3.Serra,V.,etal.,CancerRes.68(19):8022-8030(2008)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
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