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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:235kDa(complex)
Genbank#:U79143(p110α),XM_043865(p85α)
Tag(s):N-terminalGST-tag(p110α),notagonp85α
a.a:fulllength
Description:
ComplexofN-terminalGST-taggedrecombinantfull-lengthhumanp110α(GenBankAccessionNo.U79143),andrecombinantfulllength,humanp85α(notag)(GenBankAccessionNo.XM_043865).CoexpressedinaBaculovirusinfectedSf9cellexpressionsystem.p110αMW=151kDa,p85αMW=84kDa.
Synonym(s):
PIK3CA,p110alpha,Phosphoinositide-3-Kinase,Catalytic,Alpha,PIK3R1,Phosphoinositide-3-Kinase,RegulatorySubunit1(Alpha),GRB1,p85alpha
SpecificActivity:
880pmol/min/μg
UnitDefinition:
Aunitofp110α/p85αkinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofPhosphoinositol-3,4,5-Trisphosphate/minat37°C.
AssayConditions:
A25μlkinasereactionisconductedinabuffercontaining10mMTris-HCl(pH7.5),50mMNaCl,5mMMgCl2PromegaCorporation,MADIson,WI).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Vogt,PKetal.,Virology.344(1):131-(2006)
2.Bader,AGetal.,NatRevCancer.Dec5(12):921-9(2005)
2.Bader,AGetal.,NatRevCancer.Dec5(12):921-9(2005)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
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