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商品描述
Species: Mouse
Host Species: E. coli
MW: 32 kDa
Genbank #: P49772
a.a: 27–189
Background:
FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL-3, FLT-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, FLT-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.
Description:
Recombinant FLT-3 Ligand is a disulfide-linked monomer protein consisting of 164 amino acid residues, and migrates as an approximately 32 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse FLT-3 ligand mature chain was expressed in E. coli.
UniProt
P49772
Synonym(s):
Fms-Related Tyrosine Kinase 3 Ligand, FLT3L, CD135L
Purity:
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent proliferation of human AML5 cells to be in the range of 10.0 ng/ml.
Formulation:
Lyophilized from 0.2 µm filtered solution with 50 mM Tris, 100 mM NaCl, pH 8.5.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. Cancer Res. 2009 Oct 1,69(19):7747-55.
2. Blood, Jul 2009, 114: 835 - 843.
3. J. Immunol., Jun 2009, 182: 7408 - 7414.
2. Blood, Jul 2009, 114: 835 - 843.
3. J. Immunol., Jun 2009, 182: 7408 - 7414.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
GTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT ELPEPRPRQ
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
