Species: Human Host Species: Sf9 insect cells MW: 277 kDa Genbank #: NM_001093 Tag(s): C-terminal His-tag a.a: full length Description:Human acetyl-Coenzyme A carboxylase alpha transcript variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Synonym(s): acetyl-coa carboxylase, acetyl coa, acetyl coenzyme a, ACC2, acetyl-Coenzyme A carboxylase alpha transcript variant 2, ACC-2, ACC 2, ACC -2 Specific Activity: ≥20 pmoles/min/µg Assay Conditions: Assay was done using ADP-Glo (Promega) reagents with 20 μM ATP, 20 μM acetyl-CoA and 12 mM NaHCO3 in 25 μL ACC assay buffer (30 mM HEPES pH=7.4, 2 mM MgCl2, 2 mM DTT, 0.01% Brij35 and 2 mM Sodium citrate). Reaction at room temperature for 30 min. Formulation: 40 mM Tris-HCl pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 3 mM DTT, 20% glyceroland 100 mM Imidazole. Format: Aqueous buffer solution Storage / Stability:

>6 months at –80°C

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).

Application Reference(s):A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Metabolic Enzymes-Carboxylase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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