Species: Human Host Species: Sf9 insect cells MW: 131 kDa Genbank #: BC112393 Tag(s): N-terminal GST-tag a.a: full length Description:Human PDE11A (GenBank Accession No.BC112393), full length with N-terminal GST-tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system. UniProt Q9HCR9 Synonym(s): PDE11, phosphodiesterase 11A Specific Activity: ≥ 1560 pmol/min/µg Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3 , 5 -cAMP to5 -AMP per minute at 37°C. Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂ , 0.1 mg/ml BSA,200 µM cAMP, 2.5 kU 5-nucleotidase, and PDE11A at 37°C for 20 min.Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate groupwhich is detected by Malachite Green Reagent. Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM glutathione, and 3 mM DTT Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Loughney K, et al., Int. J. Impot. Res. 2005, 17(4):320-5.2. Weeks JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9.

Application Reference(s): 1.Discovery of a Novel Series of Pyrazolo[1,5-a]pyrimidine-Based Phosphodiesterase 2A Inhibitors Structurally Different from N-((1S)-1-(3-Fluoro-4-(trifluoromethoxy)phenyl)-2-methoxyethyl)-7-methoxy-2-oxo-2,3-dihydropyrido[2,3-b]pyrazine-4(1H)-carboxamide (TAK-915), for the Treatment of Cognitive Disorders(2017)2.Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions(2017)3.Design and Synthesis of Novel and Selective Phosphodiesterase 2 (PDE2a) Inhibitors for the Treatment of Memory Disorders(2017)4.Discovery of Selective Phosphodiesterase 1 Inhibitors with Memory Enhancing Properties(2017)5.Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays(2017)6.Novel N-hydroxybenzamides incorporating 2-oxoindoline with unexpected potent histone deacetylase inhibitory effects and antitumor cytotoxicity.(2017)7. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 8. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 9. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Warning(s): Avoid freeze/thaw cycles. Scientific Category: PDE Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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