PAI-1 Reporter (Luc) –Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity.
Materials Required But Not Provided:Human TGF-β1 (R+D Systems #240-B-002,)Thaw Medium 1 (BPS Cat #60187)Growth medium: Thaw Medium 1(BPS Cat #60187) + G418 500 µg/ml.Assay medium: DMEM medium (Hyclone, #SH30243.01) + 1% BSA + 1% Pen/Strep96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)ONE-STEP™ Luciferase Assay System (BPS Cat #60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer
Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO. Host Cell line: Mv1 Lu Cells Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species. Growth Media:Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)
Complete Growth Medium: Thaw Medium 1 (BPS Cat. #60187) and 500 µg/ml of Geneticin (Invitrogen, #11811031).
Instructions for use: See data sheet for detailed culturing and assay protocol. Storage / Stability: Store in liquid nitrogen immediately upon receipt. Application(s): Monitor TGF-β signaling pathway activity. Reference(s):Abe, M. et al. (1994) An assay for transforming growth factor- β using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Analytical Biochemistry 216:276-584.
Khan, S. et al. (2012) Quantification of active and total transforming growth factor-β levels in serum and solid organ tissues by bioassay. BMC Research Notes 5: 636
Notes: Contact us for pricing.License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Warning(s): Avoid freeze/thaw cycles. Scientific Category: Immunotherapy Product Type: Cell Line Data shown is lot-specific. Contact us for specific information on other lots.>>> 更多资讯详情请访问蚂蚁淘商城
