Species: Human Host Species: E. coli MW: 40.7 kDa Genbank #: NM_007371 Tag(s): N-terminal GST-tag a.a: 29-145 Description:Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 – 145 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system. UniProt Q15059 Synonym(s): Bromodomain containing 3, BRD3, RING3L Assay Conditions:

Enzyme was assayed according to BPS BRD3 (BD1) Assay Kit (Cat# 32513) protocol. BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen^®(R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol Format: Aqueous buffer solution Storage / Stability: >6 months at –80°C. Application(s): Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Reference(s): 1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Bromodomain Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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