Species: Human Host Species: Sf9 insect cells MW: 41 kDa Genbank #: NM_000459 Tag(s): N-terminal His-tag a.a: 771–end Description:Human Tie2 (GenBank Accession No. NM_000459), a.a. 771-end with N-terminal His-tag, MW=41 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Synonym(s): TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1 Specific Activity: 140 U/µg Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C. Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Tie2. Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. Format: Aqueous buffer solution Storage / Stability: >6 months at –80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Ramsden, J.D., et al., Endocrinology 146 (7), 2895-2902 (2005). 2. Tammela,T., et al., Blood 105 (12), 4642-4648 (2005). Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Receptor Tyrosine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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