Species: Human Host Species: Sf9 insect cells MW: 42.5 kDa Genbank #: NM_004972 Tag(s): N-terminal His-tag a.a: 808–end Description:Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system. Synonym(s): JAK2, Janus kinase-2, Just Another Kinase 2 Specific Activity: 118 pmol/min/μg. Assay Conditions: 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT. Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole Format: Aqueous buffer solution Storage / Stability: >6 months at –80°C Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005). 2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005). Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Cytoplasmic Tyr Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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