Species: Rat Host Species: E. coli MW: 16 kDa Genbank #: P13109 a.a: 10–154 Background:Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types. Description:Recombinant Rat bFGF is a disulfide-linked homodimeric protein consisting of 146 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat basic Fibroblast Growth Factor mature chain was expressed in E. coli. UniProt P13109 Synonym(s): bFGF, FGF2, Prostatropin, Basic Fibroblast Growth Factor, Fibroblast Growth Factor-2 Purity: ≥96% by SDS-PAGE and HPLC Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method. Biological Activity: The ED₅₀ determined by  dose-dependent stimulation of  thymidine uptake by 3T3 cells expressing FGF receptors, was found to be <1 ng/ml. Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.5. Format: lyophilized protein Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid. Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s): 1. Przybylski M. J Wound Care. 2009 Dec,18(12):516-9.2. Temple S, Qian X. Neuron. 1995 Aug,15(2):249-52. Warning(s): Avoid freeze/thaw cycles Amino Acid Sequence: P ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS Scientific Category: Cytokine/Growth Factors Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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