Species: Human Host Species: Sf9 insect cells MW: 82 kDa Genbank #: NM_002392 Tag(s): N-terminal GST-tag a.a: 2–498 Description:Mdm2 (mouse double minute 2, human homolog, also known as Hdm2), GenBank Accession No. NM_002392, (2-498) with N-terminal GST-tag, MW = 82 kDa, expressed in a baculovirus expression system. UniProt Q00987-11 Synonym(s): Mdm2,mouse double minute 2, human homolog, Hdm2 Assay Conditions: 50 µl of reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and Mdm2 (0-0.5µg) in 50 mM Tris pH 7.4) was incubated for 30 min. at 37°C. The reaction mixture was transferred to a streptavidin-coated plate and incubated for additional 30 min at 37°C. Anti-Mdm2 andHRP-labeled secondary antibody were incubated with the reaction mixture for 1 hour each, followed by chemiluminescent detection. Formulation: 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 5 mM glutathione and 10% glycerol Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Fang, S., et al. (2000). J. Biol. Chem. 275, 8945-8951.2. Chen, J et al. (1995). Mol. Med. 1, 142-152. Warning(s): Avoid freeze/thaw cycles Scientific Category: Ubiquitin E3 ligase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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