Species: Human Host Species: E. coli MW: 59 kDa Genbank #: NM_181871 Tag(s): N-terminal His-tag a.a: 2–543 (end) Background:PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. This reaction is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Description:Human pyruvate kinase variant 2 (PKL), GenBank Accession No. NM_181871, full length with N-terminal His-tag 2-543 (end), MW=59 kDa, expressed in an E. coli expression system. UniProt P30613 Synonym(s): Pyruvate Kinase RBC, PKLR Var 2, PKL, PK1 Specific Activity: ≥190,000 pmol/min/µg. Assay Conditions: A 50 µl reaction is conducted ina buffer containing 50 mM Tris (pH 7.4),10 mM MgCl₂, 50 mM KCl, 2 mM ADP, 10mM phosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo® LuminescentKinase Assay Platform. Formulation: 40 mM Tris pH 8.0, 146 mM NaCl, 20% glycerol, and 3 mM DTT Format: Aqueous buffer solution Storage / Stability:

>6 months at –80°C

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Bullido M.J. et al., Neurobiol. Aging. 29(10): 1160-6 (2008).2. Russell S.T. et al., Exp. Cell. Res. 315(1) : 16-25 (2009).

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Metabolic Enzymes-Kinase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

>>> 更多资讯详情请访问蚂蚁淘商城