Species: Human Host Species: E. coli MW: 55 kDa Genbank #: NM_004567/NP_004558 Tag(s): N-terminal His tag a.a: 2-469Description:Human recombinant 6- phosphofructo-2-kinase/fructose-2,6- bisphosphatase 4 (PFKFB4), also known as 6PF-2-K/Fru-2,6-P2ase 4, and PFK/FBpase 4, a.a. 2-469 with an Nterminal His-tag, GenBank Accession No. NM_004567/NM_004558, MW = 55 kDa, expressed in an E. coli cell expression system.UniProt Q16877Synonym(s): 6- phosphofructo-2-kinase/fructose-2,6- bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBpase 4Specific Activity: 16 pmol/min/μgAssay Conditions: The reaction mixture contained 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl₂, 1 mM
-D-fructose-6- phosphate, 20 μM ATP, 2 mM DTT, 5 mM KH₂PO₄, and various amounts of PFKFB4 in a final volume of 50 uL. The reaction was incubated at 30°C for 30 min, the amount of ATP consumed was calculated using kinase-glo plus reagent (Promega).Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200 mM imidazole and 20% glycerol.Format: Aqueous buffer solutionStorage / Stability: At least 6 months at –80°C.Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.Reference(s): 1. Minchenko, O.H., et al., Acta Biochimica Poloncia. 2005; 52(4): 881-888. 2. Goidts, V., et al., Oncogene. 2012; 31: 3235-3243.Warning(s): Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).Scientific Category: Metabolic Enzymes - KinaseProduct Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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