Species: Human Genbank #: NM_001991 (EZH1), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)Description:The EZH1 Chemiluminescent Assay Kit is designed to measure activity of theEZH1 complex (EZH1/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to the EZH1 Chemiluminescent Assay Kit isa highly specific antibody that recognizes methylated Histone H3K27. With this kit, only threesimple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionineis incubated with a sample containing assay buffer and methyltransferaseenzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeledsecondary antibody followed by addition of the HRP substrate to producechemiluminescence that can then be measured using a chemiluminescence reader.UniProt Q92800Synonym(s): KMT6B, EZHSupplied As: The EZH1 Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay Format:

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Instructions for use: See product datasheet for detailed protocol.Storage / Stability: One year from date of receipt when stored as directed.Application(s): Great for studying enzyme kinetics and HTS applications.Reference(s): 1. Dillon SC, et al. Genome Biol. 2005; 6:227. 2. Morin, RD., et al. Nat. Genet. 2010, 42(2):181.Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Rotating or rocker platformWarning(s): Avoid freeze/thaw cycles.Scientific Category: MethyltransferaseProduct Type: Assay KitData shown is lot-specific. Contact us for specific information on other lots.

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