Species: Human Host Species: E. coli MW: 22.4 kDa Genbank #: NC_004102 Tag(s): N-terminal FLAG-His-tags a.a: 21–32 of NS4A, linker (4 a.a.) and a.a. 3–181 of NS3 Description:Fusion protein corresponding to theserine protease NS3 (a.a. 3-181) andcofactor NS4A (a.a. 21-32) from HepatitisC virus genotype 1a (HCV1a), GenBankAccession No. NC_004102, with NterminalFLAG-His tag, MW = 22.4 kDa,expressed in an E. coli expressionsystem. UniProt P27958 Synonym(s): hepatitis c virus genotype 1a Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, and 250 mM imidazole. Format: Aqueous buffer solution Storage / Stability:

>6 months at –80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Lin, C., et al., Hepatitis C Virus. Norfolk (UK): Horizon Bioscience; 2006. Chapter 6. 2. Prabhu, R., et al., Exp Mol Pathol. 2004 Jun;76(3):242-52. Warning(s): Avoid freeze/thaw cycles Amino Acid Sequence: MDYKDDDDKHHHHHHGCVVIVGRIVLSGSGSITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRS Scientific Category: Protease Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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