Species: Human Host Species: Sf9 insect cells MW: 65.6 kDa Genbank #: NM_020367 Tag(s): N-terminal GST-tag a.a: 8-338 (end) Description:Human PARP11 (GenBank Accession No. NM_020367), a.a. 8-338 (end) with N-terminal GST-tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system. UniProt Q9NR21 Synonym(s): poly (ADP-ribose) polymerase 11, poly(ADP-ribosyl)transferase 11, PARP11 Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Assay Conditions: Enzyme reaction is conducted usingBPSs PARP11 Assay Kit (Catalog #80561) at room temperature for 1 hour. Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mMglutathione, 20% glycerol, and 3 mM DTT. Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62. Warning(s): Avoid freeze/thaw cycles. Scientific Category: PARP Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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