Species: Human Host Species: Sf9 insect cells MW: 118.6 kDa Genbank #: NM_145059 Tag(s): N-terminal FLAG-tag a.a: 2–1084(end) Description:Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system. UniProt Q8N0W3 Synonym(s): fucokinase, FuK Specific Activity: ≥85.3 pmole/min/µg Assay Conditions:

The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 μM ATP, and various amount of fucokinase in a final volume of 50 μL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated using Kinase-Glo Plus^® reagent (Promega).

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and 80 ng/µl FLAG peptide Format: Aqueous buffer solution Storage / Stability:

>6 months at –80°C

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Hinderlich, S., et al., Biochem Biophys Res Commun. 2002 Jun 14; 294(3):650-4.2. Zeitler, R., et al. J. Enz. Inhib. 1997 Feb; 11(4):265-73 Warning(s): Avoid freeze/thaw cycles. Scientific Category: Metabolic Enzymes-Kinase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

>>> 更多资讯详情请访问蚂蚁淘商城