Background:L-tryptophan (L-Trp) is an essential amino acid necessary for proteinsynthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established asa key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue forimmune evasion. Description:

The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is measured by measuring the absorption of reaction product at
= 320-325 nm.

Synonym(s): TDO2, tryptophan 2,3 dioxygenase Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Format:

COMPONENTS:

Instructions for use: See assay kit data sheet for detailed protocol Storage / Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Useful for the study of TDO enzymology, screening inhibitors and selectivity profiling. Reference(s):

Li, J.S., et al. Arch. Insect Biochem. Physiol. 2007 Feb; 64(2): 74–87.

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Immunotherapy Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.

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