Background:Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. Description:The PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this PD-1:PD-L1 Inhibitor Screening Assay Kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader. Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. Contraindications: DMSO >
1%, strong acids or bases, ionic detergents, high salt. Format: COMPONENTS
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s): This kit is most useful for screening for inhibitors of PD-L1. Reference(s): 1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Cell Surface Receptor/Single-pass Transmembrane Protein Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.
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