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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/Human Stromal Cell-Derived Factor-1 beta/90238-B/10 µg
商品描述
Species:Human
HostSpecies:E.coli
MW:8kDa
Genbank#:P48061
Tag(s):
a.a:22–93
Background:
SDF-1alphaandSDF-1betaaresmallcytokinesbelongingtotheCXC-Chemokines.SDF-1isidenticalwithachemokinereportedtofunctionasapre-B-cellgrowthfactorinthepresenceofIL-7andisolatedoriginallyfromamurinebonemarrowstromalcellline.HumanSDF-1alphaandSDF-1betaareencodedbyasinglegeneandarisebyalternativesplicing.SDF-1actsonlymphocytesandmonocytesbutnotneutrophilsinvitroandisahighlypotentchemoattractantformononuclearcellsinvivo.Inaddition,SDF-1alsoinducesintracellularactinpolymerizationinlymphocytes.SDFactsasachemoattractantforhumanhematopoieticProgenitorcellsexpressingCD34givingrisetomixedtypesofprogenitors,andmoreprimitivetypes.Thechemotacticresponseisinhibitedbypertussistoxin.ChemotaxisofCD34(+)cellsinresponsetoSDFisincreasedbyIL-3invitro.SDFhasbeenshownalsotoinduceatransientelevationofcytoplasmiccalciuminthesecells.
Description:
Recombinanthumanstromalcell-derivedfactor-1β(SDF1β),alsoknownasCXCL12orPre-Bcellgrowth-stimulatingfactor(PBSF),isadisulfide-linkedmonomericproteinconsistingof73a.a.andmigratesasanapproximately8kDaproteinunderreducingandnon-reducingconditions.OptimizedDNAsequenceencodinghumanSDF1βmaturechainwasexpressedinE.coli.
UniProt
P48061
Synonym(s):
stromalcell-derivedfactor-1β,SDF1β,CXCL12,Pre-Bcellgrowth-stimulatingfactor,PBSF,SDF1b,SDF-1b
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
Formulation:
Lyophilizedfroma0.2µmfilteredsolution(1mg/ml)containing20mMphosphatebuffer,pH7.0,and150mMNaCl.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/Stability:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Shirozu,M.,etal.(1995).Genomics28(3):495-500.
2.Nair,S.,Schilling,T.F.(2008).Science322(5898):89-92.
2.Nair,S.,Schilling,T.F.(2008).Science322(5898):89-92.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKRFKM
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
