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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/Human Stromal Cell-Derived Factor-1 alpha/90237-B/10 µg
商品描述
Species:Human
HostSpecies:E.coli
MW:8kDa
Genbank#:P48061
a.a:22–89
Background:
SDF-1alphaandSDF-1betaaresmallcytokinesbelongingtotheCXC-Chemokines.SDF-1isidenticalwithachemokinereportedtofunctionasapre-B-cellgrowthfactorinthepresenceofIL-7andisolatedoriginallyfromamurinebonemarrowstromalcellline.HumanSDF-1alphaandSDF-1betaareencodedbyasinglegeneandarisebyalternativesplicing.SDF-1actsonlymphocytesandmonocytesbutnotneutrophilsinvitroandisahighlypotentchemoattractantformononuclearcellsinvivo.Inaddition,SDF-1alsoinducesintracellularactinpolymerizationinlymphocytes.SDFactsasachemoattractantforhumanhematopoieticProgenitorcellsexpressingCD34givingrisetomixedtypesofprogenitors,andmoreprimitivetypes.Thechemotacticresponseisinhibitedbypertussistoxin.ChemotaxisofCD34(+)cellsinresponsetoSDFisincreasedbyIL-3invitro.SDFhasbeenshownalsotoinduceatransientelevationofcytoplasmiccalciuminthesecells.
Description:
RecombinantSDF-1alphaisadisulfide-linkedmonomerproteinconsistingof69aminoacidresidues,migratesasanapproximately8kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodinghumanStromalCell-DerivedFactor-1alphawasexpressedinE.coli.
UniProt
P48061
Synonym(s):
StromalCell-DerivedFactor-1alpha,SDF1a,CXCL12,TLSF-A,
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
Formulation:
Lyophilizedfroma0.2µmfilteredconcentrated(1mg/ml)solutionin20mMphosphatebuffer,pH7.2.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/Stability:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.HiesingerW,etal.TrendsCardiovascMed.2012Aug,22(6):139-44.
2.FelixAS,etal.CancerMicroenviron.2010Feb26,3(1):49-56.
2.FelixAS,etal.CancerMicroenviron.2010Feb26,3(1):49-56.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
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