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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/Quick PCR™ Cloning Kit without competent cells/20100/100 reactions
商品描述
Background:
TheQuickPCR™CloningKitisasimpleandhighlyefficientmethodtoinsertanygeneorDNAfragmentintoavector,withouttime-consumingligationsteps.MultiplePCRinsertscanbeaddedinasinglestep.Theprocedureiseasy:aprimersethomologoustothegeneofinterestandthevectorareusedtoamplifyaPCRproduct.ThispurifiedPCRproductisincubatedwithrestrictionenzyme-digestedvectorandReagentAoftheQuickPCR™CloningKit.Followingabrief(5minute)incubationwithReagentB,theannealedvectorisreadyfortransformation.
Description:
TheBPSBioscienceQuickPCR™cloningkitallowsfortheinsertionofmultipleDNAfragmentsintothedesiredvectorinasinglereaction.Noligationstepisrequired,savingtimeandeffort.Themethodisfast,simple,andefficient.GofromPCRproducttotransformingcellsinunderanhour!SimplymixthepurifiedPCRproductwiththevectorina1:1molarratio(TotalDNA:Vector)andfollowtheprotocol.Efficienciesof>90%areroutinelyobtained.Availablewithorwithoutcompetentcellsforevengreaterefficiencyandflexibility.
SuppliedAs:
Components:
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
Efficiency:
Efficienciesof>90%aretypical.
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Kitcomponentsmaybestoredat-20°Corbelow.Avoidfreeze/thawcycles.Uponfirstthaw,storeallreagentsinsingleusealiquots.Stableforatleast6monthsfromdateofreceipt,whenstoredasdirected.
Application(s):
KitisdesignedforcloninganygeneorDNAfragmentintoanyvectorinaquick,single-stepreaction.Canbeusedwithmultipleinsertsinasinglereaction.Alsosuitableforfixingoraddingpointmutations,insertions,ordeletions.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Mol.Biol.Reag./Cloning
ProductType:
PCRKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
