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ChIPresultsobtainedwiththeantibodydirectedagainstEZH2
ChIPassayswereperformedusingHeLacells,theantibodyagainstEZH2(Cat.#25223)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedusingshearedchromatinfrom1millioncells.Atitrationoftheantibodyconsistingof0.5,1,2.5and5µgperChIPexperimentwasanalyzed.IgG(2µg/IP)wasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersforMYT1,usedasapositivecontroltarget,andforthepromoteroftheactiveGAPDHgene,usedasanegativecontrol.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).
WesternblotanalysisusingtheantibodydirectedagainstEZH2
NuclearextractsofHeLacells(40µg)wereanalyzedbyWesternblotusingtheantibodyagainstEZH2(Cat.#25223)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterest(expectedsize85kDa)isindicatedontheright;theMarker(inkDa)isshownontheleft.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
WB(1:1000)
Immunochemistry(1:100–1:500)