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Determinationofthetiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseEHMT1(Cat.No.25220).Theplateswerecoatedwithamixofthepeptidesusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:10,600.
WesternblotanalysisusingtheantibodydirectedagainstEHMT1
NuclearextractsofHeLacells(40μg)wereanalysedbyWesternblotusingtheantibodyagainstEHMT1(Cat.No.25220)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterest(expectedsize:138kDa)isindicatedontheright;themarker(inkDa)isshownontheleft.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
WB(1:500)