WesternblotanalysisusingtheantibodydirectedagainstCHD1
WesternblotwasperformedonnuclearextractsfromHeLacells(HeLanuclearextracts,20μg)withtheantibodyagainstCHD1(Cat.No.25213)diluted1:500inTBS-Tweencontaining5%skimmedmilk.ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterestisindicatedontheright.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.