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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Background:
PRMT5isahistonemethyltransferasethatexhibitsmethylationactivitytowardH4-R3.
Description:
ThePRMT5HomogeneousAssayKitisdesignedtomeasurePRMT5activityforscreeningandprofilingapplications.PRMT5isahistonemethyltransferasethatexhibitsmethylationactivitytowardH4-R3.ThekeytothePRMT5HomogeneousAssayKitisahighlyspecificantibodythatrecognizesmethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,asamplecontainingPRMT5enzymeisincubatedwiththebiotinylatedsubstratefortwohours.Next,acceptorbeadsandprimaryantibodyareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
Synonym(s):
PRMT5,HRMT1L5,IBP72,JBP1,SKB1
SuppliedAs:
ThePRMT5HomogeneousAssayKitcomesinaconvenientAlphaLISA®format,withbiotinylatedhistoneH4peptidesubstrate,primaryantibody,methylationassaybuffer,andpurifiedPRMT5for384enzymereactions.
Contraindications:
GreenandbluedyesthatabsorblightintheAlphaScreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreen®assays.
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
Stopa,N.,etal.2015.CellMolLifeSci.72(11):2041-59.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
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