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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Background:
PARP2isknowntocatalyzetheNAD-dependentadditionofpoly(ADP-ribose)tohistones.
Description:
ThePARP2ChemiluminescentActivityAssaykitisdesignedtomeasurePARP2activityforscreeningandprofilingapplications.ThekeytothePARP2ChemiluminescentActivityAssayisthebiotinylatedsubstrate.Withthiskit,onlythreesimplestepsarerequiredforPARP2reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thePARP2biotinylatedsubstrateisincubatedwiththeassaybufferthatcontainsthePARP2enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionoftheHRPsubstratetoproduceachemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
Synonym(s):
poly(ADP-ribose)polymerase2,poly(ADP-ribosyl)transferase2,ADPRT,PARP2
SuppliedAs:
ThePARP2assaykitcomesinaconvenient96-wellformat,withpurifiedPARP2enzyme,histonemixture,activatedDNA,andPARP2assaybufferfor100enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Brown,J.A.,Marala,R.B.,J.Pharmacol.Toxicol.Methods2002;47:137-41.
ApplicationReference:
Discoveryofnovel,induced-pocketbindingoxazolidinonesaspotent,selective,andorallybioavailabletankyraseinhibitors(2013)
Notes:
Thiskitisalsosuitableforusewithcellextracts,butnonspecificribosylationactivitymaybedetected.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
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